特码最后一次大公开
首頁 ? pBI-GL四環素誘導表達Luc熒光報告載體質粒 BioVector NTCC質粒載體菌種細胞基因保藏中心

pBI-GL四環素誘導表達Luc熒光報告載體質粒 BioVector NTCC質粒載體菌種細胞基因保藏中心

  • 價  格:¥19865
  • 貨  號:pBI-GL四環素誘導表達Luc熒光報告載體質粒
  • 產  地:北京
點擊詢問我要采購
 竭誠為您服務!
BioVector NTCC典型培養物保藏中心
聯系人:Dr.Xu, Biovector NTCC Inc.點擊這里給我發消息

電話:+86-010-53513060. 工作QQ:1843439339

郵件:[email protected]

手機:18901268599

地址:北京

已注冊
 

pBI-GL四環素誘導表達Luc熒光報告載體質粒 BioVector NTCC質粒載體菌種細胞基因保藏中心
The pBI-L Tet Vector is a response plasmid that can be used to express a gene of interest
and luciferase from a bidirectional tet-responsive promoter (Pbi-1; 1) in Clontech’s Tet-On and
Tet-Off Gene Expression Systems and Cell Lines (2). The Tet Expression Systems and Cell Lines
give researchers ready access to the tetracycline-regulated expression systems described by
Gossen & Bujard (3; Tet-Off) and Gossen et al. (4; Tet-On). The pBI-L Tet Vector contains the
bidirectional promoter Pbi-1 which is responsive to the tTA and rtTA regulatory proteins in the
Tet-Off and Tet-On systems, respectively. Pbi-1 contains the Tet-responsive element (TRE), which
consists of seven copies of the 42-bp tet operator sequence (tetO). The TRE element is between
two minimal CMV promoters (PminCMV), which lack the enhancer that is part of the complete
CMV promoter. Consequently, Pbi-1 is silent in the absence of binding of TetR or rTetR to the
tetO sequences. PminCMV-1 controls the expression of the gene of interest; and PminCMV-2 controls
the expression of luciferase. Therefore, the expression of a gene of interest for which there is
no convenient assay may be monitored indirectly via the luciferase reporter function. Note that
the cloned insert must have an initiation codon. In some cases, addition of a Kozak consensus
ribosome binding site (5) may improve expression levels; however, many cDNAs have been
efficiently expressed in Tet systems without the addition of a Kozak sequence.
【Supplier來源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website網址】 http://www.biovector.net

您正在向 biovector.net  發送關于產品 pBI-GL四環素誘導表達Luc熒光報告載體質粒 BioVector NTCC質粒載體菌種細胞基因保藏中心 的詢問

點擊“立即發送”后,我們將在1個工作日內與您取得聯系。

 

特码最后一次大公开
股票涨跌百分比怎么看 90年代娃哈哈股票 股市停牌 子基金配资 海南橡胶股票 腾讯阿里巴巴股票行情 私募基金配资利率 众鑫盈配资 科创板股票涨跌幅限制 盈易点配资